RESUMO
To investigate the characteristics, source apportionment, and potential source areas of carbonaceous aerosols in Chongqing during winter, PM2.5 samples were collected from January 2021 to February 2021 in the urban areas of Wanzhou (WZ), Yubei (YB), and Shuangqiao (SQ). The results showed that the average mass concentrations of PM2.5, OC, and EC in SQ were (72.6 ±33.3), (18.2 ±8.2), and (4.4 ±1.7) µg·m-3, respectively, higher than those in WZ[(67.2 ±30.3), (17.2 ±7.4), and (5.1 ±2.4) µg·m-3] and YB[(63.4 ±25.7), (15.4 ±6.3), and (4.2 ±1.9) µg·m-3]. Compared with that during the clear period, the concentration and fraction of EC in total carbon increased by 103.0% and 8.1%, respectively, in WZ compared to that in other areas during pollution period, whereas the OC/EC ratio was decreased significantly (-10.5%), indicating that the primary emission of carbonaceous aerosols increased significantly during the pollution period. The average mass concentrations of secondary organic carbon (SOC) in SQ and YB were (7.7 ±4.8) µg·m-3 and (6.9 ±2.8) µg·m-3 significantly higher, respectively, than that in WZ[(4.5 ±1.9) µg·m-3] during the campaign. This indicated that the secondary transformation had a greater influence on the carbonaceous aerosols in SQ and YB than that in WZ. Furthermore, in contrast to that in WZ, the ratios of SOC/OC were increased with the increase in PM2.5 concentrations, and significant correlations between SOC concentration and aerosol water content, NO2 concentration, and the value of NOR were observed in SQ and YB (P < 0.01), indicating that the increasing of carbonaceous aerosol concentrations might be mainly driven by the SOC with -NO2 groups produced by aqueous chemical reactions during winter in SQ and YB. The positive definite matrix factor (PMF) results in these urban areas showed that the contribution of biomass/coal combustion source in WZ (47.4%) was significantly higher than that in YB (34.2%) and SQ (38.1%), whereas the gasoline motor vehicle emission and secondary transformation impacts were more significant in YB. The results of the concentration weighted trajectory (CWT) showed that the potential sources of carbonaceous aerosols were mainly the local and northeastern parts of these urban areas (such as Changshou).
RESUMO
BACKGROUND: Autoimmune thyroid diseases (AITDs) are chronic autoimmune diseases specific to thyroid and mainly include Graves' disease (GD) and Hashimoto' thyroiditis (HT). The adaptive immunoreactivity of CD4+ T cells plays a crucial role in the pathogenesis of AITDs, but very little has been known about its changes in disease status. METHODS: We collected peripheral CD4+ T cells from 12 GD patients, including 6 newly diagnosed GD (NGD) and 6 refractory GD (RGD) patients, 6 HT patients and 6 healthy controls, and examined the gene expression profiles and colon types of T cells receptor (TCR) ß chain complementarity determining region 3 (CDR3) using high-throughput sequencing. RESULTS: The TCR repertoire were significantly expanded in AITDs groups, and some TCR genes were expressed more preferentially in AITDs group than in the healthy control group, including TRBV15 (P = 0.001), TRBV4-2 (P = 0.003), TRBV9 (P = 0.007), TRBV3-2 (P = 0.012), TRBV7-8 (P = 0.015), TRBV25-1 (P = 0.019), TRBV12-4 (P = 0.019) and TRBV27 (P = 0.02) in GD patients as well as TRBV29-1 (P = 0.004), TRBV12-4 (P = 0.004), TRBV6-5 (P = 0.011), TRBV7-2 (P = 0.012), TRBV27 (P = 0.012), TRBV9 (P = 0.031) and TRBV4-2 (P = 0.032) in HT patients. Moreover, subgroup analysis showed that the difference in the TCR spectrum between the normal group and NGD was not obvious, but a large number of differential genes appeared in the RGD group. CONCLUSION: TCR spectrum has changed in patients with AITDs with expanded repertoire and many upregulated TRBV genes. Moreover, this difference is not apparent in GD patients at the initial stage, but as the disease progresses, the differences in TCR profiles became more pronounced.
